A report on less-severe, long-duration persistent hind-limb ischemia surgical rabbit model
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A report on less-severe, long-duration persistent hind-limb ischemia surgical rabbit model. / Harikrishnan, V. S.; Shenoy, S. J.; Ranaraj, V. R.; Sukhija, S.; Oloyo, A. K.
In: Journal of the Hellenic Veterinary Medical Society, Vol. 72, No. 4, 2021, p. 3455-3462.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A report on less-severe, long-duration persistent hind-limb ischemia surgical rabbit model
AU - Harikrishnan, V. S.
AU - Shenoy, S. J.
AU - Ranaraj, V. R.
AU - Sukhija, S.
AU - Oloyo, A. K.
N1 - Publisher Copyright: © 2021, Journal of the Hellenic Veterinary Medical Society.All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Development of a persistent hind limb ischemia rabbit model without excessive adverse symptoms and compromised animal welfare. New Zealand White (n=18, male and female) rabbits of 3-4 months of age and 3.0± 0.1 Kg body weight were used. The surgical technique for ischemia avoided the ligation of vessels above the inguinal ligament and included only the ligation and complete excision of the common and superficial femoral arteries along with all their branches up to popliteal and saphenous arteries. Study duration was 84 days. All animals completed the study period uneventfully. The activity of the animals remained unaffected throughout the study except for the first post-operative day. Adverse symptoms of other models such as loss of limb due to necrosis, loss of nails and necrosis of skin were not observed while successful ischemia was confirmed. There was a significant decrease (P=0.0381) in ischemic right limb circumference.Terminal angiography by abdominal aortic cannulation in the animals demonstrated negligible amount of angiogenesis at the distal ischemic thigh in comparison to the control limb (P=0.001). This study reports successful development of a refined chronic hindlimb ischemia rabbit model
AB - Development of a persistent hind limb ischemia rabbit model without excessive adverse symptoms and compromised animal welfare. New Zealand White (n=18, male and female) rabbits of 3-4 months of age and 3.0± 0.1 Kg body weight were used. The surgical technique for ischemia avoided the ligation of vessels above the inguinal ligament and included only the ligation and complete excision of the common and superficial femoral arteries along with all their branches up to popliteal and saphenous arteries. Study duration was 84 days. All animals completed the study period uneventfully. The activity of the animals remained unaffected throughout the study except for the first post-operative day. Adverse symptoms of other models such as loss of limb due to necrosis, loss of nails and necrosis of skin were not observed while successful ischemia was confirmed. There was a significant decrease (P=0.0381) in ischemic right limb circumference.Terminal angiography by abdominal aortic cannulation in the animals demonstrated negligible amount of angiogenesis at the distal ischemic thigh in comparison to the control limb (P=0.001). This study reports successful development of a refined chronic hindlimb ischemia rabbit model
KW - Angiography
KW - Hind limb ischemia
KW - Rabbit model
KW - Thigh muscle atrophy
KW - Welfare
U2 - 10.12681/jhvms.29395
DO - 10.12681/jhvms.29395
M3 - Journal article
AN - SCOPUS:85125103930
VL - 72
SP - 3455
EP - 3462
JO - Journal of the Hellenic Veterinary Medical Society
JF - Journal of the Hellenic Veterinary Medical Society
SN - 1792-2720
IS - 4
ER -
ID: 306678230