Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain

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Standard

Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. / Harikrishnan, V. S.; Palekkodan, Hamza; Fasaludeen, Ansar; Krishnan, Lissy K.; Abelson, Klas S.P.

In: Heliyon, Vol. 7, No. 7, e07500, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Harikrishnan, VS, Palekkodan, H, Fasaludeen, A, Krishnan, LK & Abelson, KSP 2021, 'Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain', Heliyon, vol. 7, no. 7, e07500. https://doi.org/10.1016/j.heliyon.2021.e07500

APA

Harikrishnan, V. S., Palekkodan, H., Fasaludeen, A., Krishnan, L. K., & Abelson, K. S. P. (2021). Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon, 7(7), [e07500]. https://doi.org/10.1016/j.heliyon.2021.e07500

Vancouver

Harikrishnan VS, Palekkodan H, Fasaludeen A, Krishnan LK, Abelson KSP. Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon. 2021;7(7). e07500. https://doi.org/10.1016/j.heliyon.2021.e07500

Author

Harikrishnan, V. S. ; Palekkodan, Hamza ; Fasaludeen, Ansar ; Krishnan, Lissy K. ; Abelson, Klas S.P. / Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. In: Heliyon. 2021 ; Vol. 7, No. 7.

Bibtex

@article{3c3d7fa58e1b46a78d74fab9f1de3a7d,
title = "Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain",
abstract = "Background: Laminectomy produces trauma in spinal cord injury (SCI) animal models resulting in impinging artefacts and welfare issues. Mechanizing laminectomy using a dental burr assisted (DBA) technique to reduce the impact of conventionally performed laminectomy on animal welfare without any alterations in the outcome of the model was previously demonstrated. However, further validation was necessary to establish it as an alternative in developing SCI rats as a model of chronic pain and memory loss. Novel method: DBA technique was employed to perform laminectomy at T10-T11 vertebrae in rats undergoing contusion SCI as a model of chronic pain and memory loss. In a 56-day study, 24 female Wistar rats (Crl: WI) were assigned randomly to four equal groups: conventionally laminectomised, DBA laminectomised, conventionally laminectomised with SCI and DBA laminectomised with SCI. Results: The study revealed DBA technique to cause less surgical bleeding (p = 0.001), lower Rat Grimace Scale (p = 0.0006); resulted in better body weight changes (p = 0.0002 on Day 7 and p = 0.0108 on Day 28) and dark phase activity (p = .0.0014 on Day 1; p = 0.0422 on Day 56). Different techniques did not differ in Basso Beattie Bresnahan score, novel object recognition, mechanical allodynia, number of surviving neurons and the area of vacuolation- indicating that the new method doesn't affect the validity of the model. Comparison with existing method(s): In comparison with the conventional technique, motorised laminectomy can be a valid tool that evokes lesser pain and ensures higher well-being in rats modelled for chronic pain and memory loss. Conclusions: The intended outcome from the model is not influenced by techniques whereas the DBA-technique is a refined alternative to the conventional method in achieving better welfare in SCI studies.",
keywords = "BBB score, Chronic pain, Dental burr assisted laminectomy, Memory loss, Rat model, Refinement, Spinal cord injury",
author = "Harikrishnan, {V. S.} and Hamza Palekkodan and Ansar Fasaludeen and Krishnan, {Lissy K.} and Abelson, {Klas S.P.}",
note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
doi = "10.1016/j.heliyon.2021.e07500",
language = "English",
volume = "7",
journal = "Heliyon",
issn = "2405-8440",
publisher = "Elsevier",
number = "7",

}

RIS

TY - JOUR

T1 - Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain

AU - Harikrishnan, V. S.

AU - Palekkodan, Hamza

AU - Fasaludeen, Ansar

AU - Krishnan, Lissy K.

AU - Abelson, Klas S.P.

N1 - Publisher Copyright: © 2021 The Author(s)

PY - 2021

Y1 - 2021

N2 - Background: Laminectomy produces trauma in spinal cord injury (SCI) animal models resulting in impinging artefacts and welfare issues. Mechanizing laminectomy using a dental burr assisted (DBA) technique to reduce the impact of conventionally performed laminectomy on animal welfare without any alterations in the outcome of the model was previously demonstrated. However, further validation was necessary to establish it as an alternative in developing SCI rats as a model of chronic pain and memory loss. Novel method: DBA technique was employed to perform laminectomy at T10-T11 vertebrae in rats undergoing contusion SCI as a model of chronic pain and memory loss. In a 56-day study, 24 female Wistar rats (Crl: WI) were assigned randomly to four equal groups: conventionally laminectomised, DBA laminectomised, conventionally laminectomised with SCI and DBA laminectomised with SCI. Results: The study revealed DBA technique to cause less surgical bleeding (p = 0.001), lower Rat Grimace Scale (p = 0.0006); resulted in better body weight changes (p = 0.0002 on Day 7 and p = 0.0108 on Day 28) and dark phase activity (p = .0.0014 on Day 1; p = 0.0422 on Day 56). Different techniques did not differ in Basso Beattie Bresnahan score, novel object recognition, mechanical allodynia, number of surviving neurons and the area of vacuolation- indicating that the new method doesn't affect the validity of the model. Comparison with existing method(s): In comparison with the conventional technique, motorised laminectomy can be a valid tool that evokes lesser pain and ensures higher well-being in rats modelled for chronic pain and memory loss. Conclusions: The intended outcome from the model is not influenced by techniques whereas the DBA-technique is a refined alternative to the conventional method in achieving better welfare in SCI studies.

AB - Background: Laminectomy produces trauma in spinal cord injury (SCI) animal models resulting in impinging artefacts and welfare issues. Mechanizing laminectomy using a dental burr assisted (DBA) technique to reduce the impact of conventionally performed laminectomy on animal welfare without any alterations in the outcome of the model was previously demonstrated. However, further validation was necessary to establish it as an alternative in developing SCI rats as a model of chronic pain and memory loss. Novel method: DBA technique was employed to perform laminectomy at T10-T11 vertebrae in rats undergoing contusion SCI as a model of chronic pain and memory loss. In a 56-day study, 24 female Wistar rats (Crl: WI) were assigned randomly to four equal groups: conventionally laminectomised, DBA laminectomised, conventionally laminectomised with SCI and DBA laminectomised with SCI. Results: The study revealed DBA technique to cause less surgical bleeding (p = 0.001), lower Rat Grimace Scale (p = 0.0006); resulted in better body weight changes (p = 0.0002 on Day 7 and p = 0.0108 on Day 28) and dark phase activity (p = .0.0014 on Day 1; p = 0.0422 on Day 56). Different techniques did not differ in Basso Beattie Bresnahan score, novel object recognition, mechanical allodynia, number of surviving neurons and the area of vacuolation- indicating that the new method doesn't affect the validity of the model. Comparison with existing method(s): In comparison with the conventional technique, motorised laminectomy can be a valid tool that evokes lesser pain and ensures higher well-being in rats modelled for chronic pain and memory loss. Conclusions: The intended outcome from the model is not influenced by techniques whereas the DBA-technique is a refined alternative to the conventional method in achieving better welfare in SCI studies.

KW - BBB score

KW - Chronic pain

KW - Dental burr assisted laminectomy

KW - Memory loss

KW - Rat model

KW - Refinement

KW - Spinal cord injury

U2 - 10.1016/j.heliyon.2021.e07500

DO - 10.1016/j.heliyon.2021.e07500

M3 - Journal article

C2 - 34286145

AN - SCOPUS:85109428514

VL - 7

JO - Heliyon

JF - Heliyon

SN - 2405-8440

IS - 7

M1 - e07500

ER -

ID: 280290361